I just thought that it was interesting to note that the gemara consistenly refers to Shmuel's sisters as 'the daughters of Shmuel's father' instead of just saying 'Shmuel's sisters.'
Also, Shmuel's father prohibited his daughters from sleeping in the same bed, because he was worried that if they engaged in homosexual acts, they would become assur to the kohen gadol. Why was this his worry? The beit hamikdash wasn't around then! All tosfot says is that he was 'machmir.'
Does anyone have any other insights into this?